dna standards Search Results


nist stool matrix  (ATCC)
93
ATCC nist stool matrix
Stool samples collected from omnivorous donors and processed into a single standardized matrix by <t>NIST</t> was spiked with ATCC CoV-2 RNA or BCoV vaccine. Spiked stool was preserved in the OMNIgene-GUT Kit (OG), Zymo DNA/RNA shield buffer (ZY), and PBS (as indicated in the tab on the top). RNA was extracted from these samples by two independent users, each in duplicate, using the MagMAX Viral/Pathogen Kit (MM; green), QIAamp Viral RNA Mini Kit (QA; orange), or Zymo Quick-RNA Viral Kit (ZY; purple) as indicated on the x -axis. RNA was assayed using ddPCR. a Absolute concentration of SARS-CoV-2 RNA assayed by ddPCR targeting the N1 gene is plotted on the y -axis. NIST stool matrix was spiked with 10 <t>3</t> <t>(triangle)</t> or 10 4 (square) copies of ATCC synthetic SARS-CoV-2 RNA. b Absolute concentration of BCoV RNA assayed by ddPCR targeting the M gene is plotted on the y -axis. NIST stool matrix was spiked with 1:10 diluted (triangle) or undiluted (square) BCoV vaccine. Control samples with no spiked in RNA (none; circle) were included in duplicate to estimate LoB. U stands for undetermined and marks samples with no detectable RNA above LoB. Two-sided paired T tests were performed on n = 4 independent extractions for each spike-in condition. Associated statistics are summarized in Supplementary Data . Source data are provided as a Source data file.
Nist Stool Matrix, supplied by ATCC, used in various techniques. Bioz Stars score: 93/100, based on 1 PubMed citations. ZERO BIAS - scores, article reviews, protocol conditions and more
https://www.bioz.com/product/dna+standards/NIST+Standard+Reference+Material+Genomic+DNA+Standards+for+Her2+Measurements%3BHuman/pmc08486790-90-0-15
Average 93 stars, based on 1 article reviews
nist stool matrix - by Bioz Stars, 2026-11
93/100 stars
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neb taq dna polymerase  (New England Biolabs)
98
New England Biolabs neb taq dna polymerase
Stool samples collected from omnivorous donors and processed into a single standardized matrix by <t>NIST</t> was spiked with ATCC CoV-2 RNA or BCoV vaccine. Spiked stool was preserved in the OMNIgene-GUT Kit (OG), Zymo DNA/RNA shield buffer (ZY), and PBS (as indicated in the tab on the top). RNA was extracted from these samples by two independent users, each in duplicate, using the MagMAX Viral/Pathogen Kit (MM; green), QIAamp Viral RNA Mini Kit (QA; orange), or Zymo Quick-RNA Viral Kit (ZY; purple) as indicated on the x -axis. RNA was assayed using ddPCR. a Absolute concentration of SARS-CoV-2 RNA assayed by ddPCR targeting the N1 gene is plotted on the y -axis. NIST stool matrix was spiked with 10 <t>3</t> <t>(triangle)</t> or 10 4 (square) copies of ATCC synthetic SARS-CoV-2 RNA. b Absolute concentration of BCoV RNA assayed by ddPCR targeting the M gene is plotted on the y -axis. NIST stool matrix was spiked with 1:10 diluted (triangle) or undiluted (square) BCoV vaccine. Control samples with no spiked in RNA (none; circle) were included in duplicate to estimate LoB. U stands for undetermined and marks samples with no detectable RNA above LoB. Two-sided paired T tests were performed on n = 4 independent extractions for each spike-in condition. Associated statistics are summarized in Supplementary Data . Source data are provided as a Source data file.
Neb Taq Dna Polymerase, supplied by New England Biolabs, used in various techniques. Bioz Stars score: 98/100, based on 1 PubMed citations. ZERO BIAS - scores, article reviews, protocol conditions and more
https://www.bioz.com/product/dna+standards/Taq+DNA+Polymerase+with+Standard+Taq+(Mg/bio_rxiv__64898__2026__03__02__709049-74-12-12
Average 98 stars, based on 1 article reviews
neb taq dna polymerase - by Bioz Stars, 2026-11
98/100 stars
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chef dna size standards  (Bio-Rad)
94
Bio-Rad chef dna size standards
Stool samples collected from omnivorous donors and processed into a single standardized matrix by <t>NIST</t> was spiked with ATCC CoV-2 RNA or BCoV vaccine. Spiked stool was preserved in the OMNIgene-GUT Kit (OG), Zymo DNA/RNA shield buffer (ZY), and PBS (as indicated in the tab on the top). RNA was extracted from these samples by two independent users, each in duplicate, using the MagMAX Viral/Pathogen Kit (MM; green), QIAamp Viral RNA Mini Kit (QA; orange), or Zymo Quick-RNA Viral Kit (ZY; purple) as indicated on the x -axis. RNA was assayed using ddPCR. a Absolute concentration of SARS-CoV-2 RNA assayed by ddPCR targeting the N1 gene is plotted on the y -axis. NIST stool matrix was spiked with 10 <t>3</t> <t>(triangle)</t> or 10 4 (square) copies of ATCC synthetic SARS-CoV-2 RNA. b Absolute concentration of BCoV RNA assayed by ddPCR targeting the M gene is plotted on the y -axis. NIST stool matrix was spiked with 1:10 diluted (triangle) or undiluted (square) BCoV vaccine. Control samples with no spiked in RNA (none; circle) were included in duplicate to estimate LoB. U stands for undetermined and marks samples with no detectable RNA above LoB. Two-sided paired T tests were performed on n = 4 independent extractions for each spike-in condition. Associated statistics are summarized in Supplementary Data . Source data are provided as a Source data file.
Chef Dna Size Standards, supplied by Bio-Rad, used in various techniques. Bioz Stars score: 94/100, based on 1 PubMed citations. ZERO BIAS - scores, article reviews, protocol conditions and more
https://www.bioz.com/product/dna+standards/CHEF+DNA+Size+Standard/10__1128_slash_aem__02548___16-78-16-23
Average 94 stars, based on 1 article reviews
chef dna size standards - by Bioz Stars, 2026-11
94/100 stars
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human methylated dna  (Zymo Research)
95
Zymo Research human methylated dna
Stool samples collected from omnivorous donors and processed into a single standardized matrix by <t>NIST</t> was spiked with ATCC CoV-2 RNA or BCoV vaccine. Spiked stool was preserved in the OMNIgene-GUT Kit (OG), Zymo DNA/RNA shield buffer (ZY), and PBS (as indicated in the tab on the top). RNA was extracted from these samples by two independent users, each in duplicate, using the MagMAX Viral/Pathogen Kit (MM; green), QIAamp Viral RNA Mini Kit (QA; orange), or Zymo Quick-RNA Viral Kit (ZY; purple) as indicated on the x -axis. RNA was assayed using ddPCR. a Absolute concentration of SARS-CoV-2 RNA assayed by ddPCR targeting the N1 gene is plotted on the y -axis. NIST stool matrix was spiked with 10 <t>3</t> <t>(triangle)</t> or 10 4 (square) copies of ATCC synthetic SARS-CoV-2 RNA. b Absolute concentration of BCoV RNA assayed by ddPCR targeting the M gene is plotted on the y -axis. NIST stool matrix was spiked with 1:10 diluted (triangle) or undiluted (square) BCoV vaccine. Control samples with no spiked in RNA (none; circle) were included in duplicate to estimate LoB. U stands for undetermined and marks samples with no detectable RNA above LoB. Two-sided paired T tests were performed on n = 4 independent extractions for each spike-in condition. Associated statistics are summarized in Supplementary Data . Source data are provided as a Source data file.
Human Methylated Dna, supplied by Zymo Research, used in various techniques. Bioz Stars score: 95/100, based on 1 PubMed citations. ZERO BIAS - scores, article reviews, protocol conditions and more
https://www.bioz.com/product/dna+standards/Universal+Methylated+Human+DNA+Standard/pm41871060-70-0-3
Average 95 stars, based on 1 article reviews
human methylated dna - by Bioz Stars, 2026-11
95/100 stars
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community  (Zymo Research)
96
Zymo Research community
Stool samples collected from omnivorous donors and processed into a single standardized matrix by <t>NIST</t> was spiked with ATCC CoV-2 RNA or BCoV vaccine. Spiked stool was preserved in the OMNIgene-GUT Kit (OG), Zymo DNA/RNA shield buffer (ZY), and PBS (as indicated in the tab on the top). RNA was extracted from these samples by two independent users, each in duplicate, using the MagMAX Viral/Pathogen Kit (MM; green), QIAamp Viral RNA Mini Kit (QA; orange), or Zymo Quick-RNA Viral Kit (ZY; purple) as indicated on the x -axis. RNA was assayed using ddPCR. a Absolute concentration of SARS-CoV-2 RNA assayed by ddPCR targeting the N1 gene is plotted on the y -axis. NIST stool matrix was spiked with 10 <t>3</t> <t>(triangle)</t> or 10 4 (square) copies of ATCC synthetic SARS-CoV-2 RNA. b Absolute concentration of BCoV RNA assayed by ddPCR targeting the M gene is plotted on the y -axis. NIST stool matrix was spiked with 1:10 diluted (triangle) or undiluted (square) BCoV vaccine. Control samples with no spiked in RNA (none; circle) were included in duplicate to estimate LoB. U stands for undetermined and marks samples with no detectable RNA above LoB. Two-sided paired T tests were performed on n = 4 independent extractions for each spike-in condition. Associated statistics are summarized in Supplementary Data . Source data are provided as a Source data file.
Community, supplied by Zymo Research, used in various techniques. Bioz Stars score: 96/100, based on 1 PubMed citations. ZERO BIAS - scores, article reviews, protocol conditions and more
https://www.bioz.com/product/dna+standards/ZymoBIOMICS+Microbial+Community+DNA+Standard/med_rxiv__64898__2026__04__18__26351176-83-4-10
Average 96 stars, based on 1 article reviews
community - by Bioz Stars, 2026-11
96/100 stars
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cfdna pan cancer reference standards  (Twist Bioscience)
94
Twist Bioscience cfdna pan cancer reference standards
Stool samples collected from omnivorous donors and processed into a single standardized matrix by <t>NIST</t> was spiked with ATCC CoV-2 RNA or BCoV vaccine. Spiked stool was preserved in the OMNIgene-GUT Kit (OG), Zymo DNA/RNA shield buffer (ZY), and PBS (as indicated in the tab on the top). RNA was extracted from these samples by two independent users, each in duplicate, using the MagMAX Viral/Pathogen Kit (MM; green), QIAamp Viral RNA Mini Kit (QA; orange), or Zymo Quick-RNA Viral Kit (ZY; purple) as indicated on the x -axis. RNA was assayed using ddPCR. a Absolute concentration of SARS-CoV-2 RNA assayed by ddPCR targeting the N1 gene is plotted on the y -axis. NIST stool matrix was spiked with 10 <t>3</t> <t>(triangle)</t> or 10 4 (square) copies of ATCC synthetic SARS-CoV-2 RNA. b Absolute concentration of BCoV RNA assayed by ddPCR targeting the M gene is plotted on the y -axis. NIST stool matrix was spiked with 1:10 diluted (triangle) or undiluted (square) BCoV vaccine. Control samples with no spiked in RNA (none; circle) were included in duplicate to estimate LoB. U stands for undetermined and marks samples with no detectable RNA above LoB. Two-sided paired T tests were performed on n = 4 independent extractions for each spike-in condition. Associated statistics are summarized in Supplementary Data . Source data are provided as a Source data file.
Cfdna Pan Cancer Reference Standards, supplied by Twist Bioscience, used in various techniques. Bioz Stars score: 94/100, based on 1 PubMed citations. ZERO BIAS - scores, article reviews, protocol conditions and more
https://www.bioz.com/product/dna+standards/Twist+cfDNA+Pan-cancer+Reference+Standards%2C+Early+Access/pm40849662-88-34-33
Average 94 stars, based on 1 article reviews
cfdna pan cancer reference standards - by Bioz Stars, 2026-11
94/100 stars
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5 hydroxymethylcytosine dna standard set  (Zymo Research)
94
Zymo Research 5 hydroxymethylcytosine dna standard set
Stool samples collected from omnivorous donors and processed into a single standardized matrix by <t>NIST</t> was spiked with ATCC CoV-2 RNA or BCoV vaccine. Spiked stool was preserved in the OMNIgene-GUT Kit (OG), Zymo DNA/RNA shield buffer (ZY), and PBS (as indicated in the tab on the top). RNA was extracted from these samples by two independent users, each in duplicate, using the MagMAX Viral/Pathogen Kit (MM; green), QIAamp Viral RNA Mini Kit (QA; orange), or Zymo Quick-RNA Viral Kit (ZY; purple) as indicated on the x -axis. RNA was assayed using ddPCR. a Absolute concentration of SARS-CoV-2 RNA assayed by ddPCR targeting the N1 gene is plotted on the y -axis. NIST stool matrix was spiked with 10 <t>3</t> <t>(triangle)</t> or 10 4 (square) copies of ATCC synthetic SARS-CoV-2 RNA. b Absolute concentration of BCoV RNA assayed by ddPCR targeting the M gene is plotted on the y -axis. NIST stool matrix was spiked with 1:10 diluted (triangle) or undiluted (square) BCoV vaccine. Control samples with no spiked in RNA (none; circle) were included in duplicate to estimate LoB. U stands for undetermined and marks samples with no detectable RNA above LoB. Two-sided paired T tests were performed on n = 4 independent extractions for each spike-in condition. Associated statistics are summarized in Supplementary Data . Source data are provided as a Source data file.
5 Hydroxymethylcytosine Dna Standard Set, supplied by Zymo Research, used in various techniques. Bioz Stars score: 94/100, based on 1 PubMed citations. ZERO BIAS - scores, article reviews, protocol conditions and more
https://www.bioz.com/product/dna+standards/5-Methylcytosine+%26+5-Hydroxymethylcytosine+DNA+Standard+Set/pm42017455-88-13-17
Average 94 stars, based on 1 article reviews
5 hydroxymethylcytosine dna standard set - by Bioz Stars, 2026-11
94/100 stars
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dna size marker  (Rockland Immunochemicals)
85
Rockland Immunochemicals dna size marker
Stool samples collected from omnivorous donors and processed into a single standardized matrix by <t>NIST</t> was spiked with ATCC CoV-2 RNA or BCoV vaccine. Spiked stool was preserved in the OMNIgene-GUT Kit (OG), Zymo DNA/RNA shield buffer (ZY), and PBS (as indicated in the tab on the top). RNA was extracted from these samples by two independent users, each in duplicate, using the MagMAX Viral/Pathogen Kit (MM; green), QIAamp Viral RNA Mini Kit (QA; orange), or Zymo Quick-RNA Viral Kit (ZY; purple) as indicated on the x -axis. RNA was assayed using ddPCR. a Absolute concentration of SARS-CoV-2 RNA assayed by ddPCR targeting the N1 gene is plotted on the y -axis. NIST stool matrix was spiked with 10 <t>3</t> <t>(triangle)</t> or 10 4 (square) copies of ATCC synthetic SARS-CoV-2 RNA. b Absolute concentration of BCoV RNA assayed by ddPCR targeting the M gene is plotted on the y -axis. NIST stool matrix was spiked with 1:10 diluted (triangle) or undiluted (square) BCoV vaccine. Control samples with no spiked in RNA (none; circle) were included in duplicate to estimate LoB. U stands for undetermined and marks samples with no detectable RNA above LoB. Two-sided paired T tests were performed on n = 4 independent extractions for each spike-in condition. Associated statistics are summarized in Supplementary Data . Source data are provided as a Source data file.
Dna Size Marker, supplied by Rockland Immunochemicals, used in various techniques. Bioz Stars score: 85/100, based on 1 PubMed citations. ZERO BIAS - scores, article reviews, protocol conditions and more
https://www.bioz.com/product/dna+standards/Diamond+DNA+Ladder+100-10Kbp/10__1158_slash_1078___0432__ccr___04___2333-57-24-39
Average 85 stars, based on 1 article reviews
dna size marker - by Bioz Stars, 2026-11
85/100 stars
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methylated mouse dna standard  (Zymo Research)
95
Zymo Research methylated mouse dna standard
Stool samples collected from omnivorous donors and processed into a single standardized matrix by <t>NIST</t> was spiked with ATCC CoV-2 RNA or BCoV vaccine. Spiked stool was preserved in the OMNIgene-GUT Kit (OG), Zymo DNA/RNA shield buffer (ZY), and PBS (as indicated in the tab on the top). RNA was extracted from these samples by two independent users, each in duplicate, using the MagMAX Viral/Pathogen Kit (MM; green), QIAamp Viral RNA Mini Kit (QA; orange), or Zymo Quick-RNA Viral Kit (ZY; purple) as indicated on the x -axis. RNA was assayed using ddPCR. a Absolute concentration of SARS-CoV-2 RNA assayed by ddPCR targeting the N1 gene is plotted on the y -axis. NIST stool matrix was spiked with 10 <t>3</t> <t>(triangle)</t> or 10 4 (square) copies of ATCC synthetic SARS-CoV-2 RNA. b Absolute concentration of BCoV RNA assayed by ddPCR targeting the M gene is plotted on the y -axis. NIST stool matrix was spiked with 1:10 diluted (triangle) or undiluted (square) BCoV vaccine. Control samples with no spiked in RNA (none; circle) were included in duplicate to estimate LoB. U stands for undetermined and marks samples with no detectable RNA above LoB. Two-sided paired T tests were performed on n = 4 independent extractions for each spike-in condition. Associated statistics are summarized in Supplementary Data . Source data are provided as a Source data file.
Methylated Mouse Dna Standard, supplied by Zymo Research, used in various techniques. Bioz Stars score: 95/100, based on 1 PubMed citations. ZERO BIAS - scores, article reviews, protocol conditions and more
https://www.bioz.com/product/dna+standards/Universal+Methylated+Mouse+DNA+Standard/pm41757999-159-50-54
Average 95 stars, based on 1 article reviews
methylated mouse dna standard - by Bioz Stars, 2026-11
95/100 stars
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technology reference materials 8391  (National Institute of Standards and Technology)
90
National Institute of Standards and Technology technology reference materials 8391
Stool samples collected from omnivorous donors and processed into a single standardized matrix by <t>NIST</t> was spiked with ATCC CoV-2 RNA or BCoV vaccine. Spiked stool was preserved in the OMNIgene-GUT Kit (OG), Zymo DNA/RNA shield buffer (ZY), and PBS (as indicated in the tab on the top). RNA was extracted from these samples by two independent users, each in duplicate, using the MagMAX Viral/Pathogen Kit (MM; green), QIAamp Viral RNA Mini Kit (QA; orange), or Zymo Quick-RNA Viral Kit (ZY; purple) as indicated on the x -axis. RNA was assayed using ddPCR. a Absolute concentration of SARS-CoV-2 RNA assayed by ddPCR targeting the N1 gene is plotted on the y -axis. NIST stool matrix was spiked with 10 <t>3</t> <t>(triangle)</t> or 10 4 (square) copies of ATCC synthetic SARS-CoV-2 RNA. b Absolute concentration of BCoV RNA assayed by ddPCR targeting the M gene is plotted on the y -axis. NIST stool matrix was spiked with 1:10 diluted (triangle) or undiluted (square) BCoV vaccine. Control samples with no spiked in RNA (none; circle) were included in duplicate to estimate LoB. U stands for undetermined and marks samples with no detectable RNA above LoB. Two-sided paired T tests were performed on n = 4 independent extractions for each spike-in condition. Associated statistics are summarized in Supplementary Data . Source data are provided as a Source data file.
Technology Reference Materials 8391, supplied by National Institute of Standards and Technology, used in various techniques. Bioz Stars score: 90/100, based on 1 PubMed citations. ZERO BIAS - scores, article reviews, protocol conditions and more
https://www.bioz.com/product/dna+standards/Human+DNA+for+Whole-Genome+Variant+Assessment/10__1016_slash_j__xgen__2022__100128-155-27-22
Average 90 stars, based on 1 article reviews
technology reference materials 8391 - by Bioz Stars, 2026-11
90/100 stars
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twist cfdna  (Twist Bioscience)
93
Twist Bioscience twist cfdna
Stool samples collected from omnivorous donors and processed into a single standardized matrix by <t>NIST</t> was spiked with ATCC CoV-2 RNA or BCoV vaccine. Spiked stool was preserved in the OMNIgene-GUT Kit (OG), Zymo DNA/RNA shield buffer (ZY), and PBS (as indicated in the tab on the top). RNA was extracted from these samples by two independent users, each in duplicate, using the MagMAX Viral/Pathogen Kit (MM; green), QIAamp Viral RNA Mini Kit (QA; orange), or Zymo Quick-RNA Viral Kit (ZY; purple) as indicated on the x -axis. RNA was assayed using ddPCR. a Absolute concentration of SARS-CoV-2 RNA assayed by ddPCR targeting the N1 gene is plotted on the y -axis. NIST stool matrix was spiked with 10 <t>3</t> <t>(triangle)</t> or 10 4 (square) copies of ATCC synthetic SARS-CoV-2 RNA. b Absolute concentration of BCoV RNA assayed by ddPCR targeting the M gene is plotted on the y -axis. NIST stool matrix was spiked with 1:10 diluted (triangle) or undiluted (square) BCoV vaccine. Control samples with no spiked in RNA (none; circle) were included in duplicate to estimate LoB. U stands for undetermined and marks samples with no detectable RNA above LoB. Two-sided paired T tests were performed on n = 4 independent extractions for each spike-in condition. Associated statistics are summarized in Supplementary Data . Source data are provided as a Source data file.
Twist Cfdna, supplied by Twist Bioscience, used in various techniques. Bioz Stars score: 93/100, based on 1 PubMed citations. ZERO BIAS - scores, article reviews, protocol conditions and more
https://www.bioz.com/product/dna+standards/Twist+cfDNA+Pan-cancer+Reference+Standard+5%25/pm40849662-140-11-11
Average 93 stars, based on 1 article reviews
twist cfdna - by Bioz Stars, 2026-11
93/100 stars
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zymobiomics hmw dna standard  (Zymo Research)
95
Zymo Research zymobiomics hmw dna standard
Stool samples collected from omnivorous donors and processed into a single standardized matrix by <t>NIST</t> was spiked with ATCC CoV-2 RNA or BCoV vaccine. Spiked stool was preserved in the OMNIgene-GUT Kit (OG), Zymo DNA/RNA shield buffer (ZY), and PBS (as indicated in the tab on the top). RNA was extracted from these samples by two independent users, each in duplicate, using the MagMAX Viral/Pathogen Kit (MM; green), QIAamp Viral RNA Mini Kit (QA; orange), or Zymo Quick-RNA Viral Kit (ZY; purple) as indicated on the x -axis. RNA was assayed using ddPCR. a Absolute concentration of SARS-CoV-2 RNA assayed by ddPCR targeting the N1 gene is plotted on the y -axis. NIST stool matrix was spiked with 10 <t>3</t> <t>(triangle)</t> or 10 4 (square) copies of ATCC synthetic SARS-CoV-2 RNA. b Absolute concentration of BCoV RNA assayed by ddPCR targeting the M gene is plotted on the y -axis. NIST stool matrix was spiked with 1:10 diluted (triangle) or undiluted (square) BCoV vaccine. Control samples with no spiked in RNA (none; circle) were included in duplicate to estimate LoB. U stands for undetermined and marks samples with no detectable RNA above LoB. Two-sided paired T tests were performed on n = 4 independent extractions for each spike-in condition. Associated statistics are summarized in Supplementary Data . Source data are provided as a Source data file.
Zymobiomics Hmw Dna Standard, supplied by Zymo Research, used in various techniques. Bioz Stars score: 95/100, based on 1 PubMed citations. ZERO BIAS - scores, article reviews, protocol conditions and more
https://www.bioz.com/product/dna+standards/ZymoBIOMICS+HMW+DNA+Standard/pm41805176-85-4-9
Average 95 stars, based on 1 article reviews
zymobiomics hmw dna standard - by Bioz Stars, 2026-11
95/100 stars
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Image Search Results


Stool samples collected from omnivorous donors and processed into a single standardized matrix by NIST was spiked with ATCC CoV-2 RNA or BCoV vaccine. Spiked stool was preserved in the OMNIgene-GUT Kit (OG), Zymo DNA/RNA shield buffer (ZY), and PBS (as indicated in the tab on the top). RNA was extracted from these samples by two independent users, each in duplicate, using the MagMAX Viral/Pathogen Kit (MM; green), QIAamp Viral RNA Mini Kit (QA; orange), or Zymo Quick-RNA Viral Kit (ZY; purple) as indicated on the x -axis. RNA was assayed using ddPCR. a Absolute concentration of SARS-CoV-2 RNA assayed by ddPCR targeting the N1 gene is plotted on the y -axis. NIST stool matrix was spiked with 10 3 (triangle) or 10 4 (square) copies of ATCC synthetic SARS-CoV-2 RNA. b Absolute concentration of BCoV RNA assayed by ddPCR targeting the M gene is plotted on the y -axis. NIST stool matrix was spiked with 1:10 diluted (triangle) or undiluted (square) BCoV vaccine. Control samples with no spiked in RNA (none; circle) were included in duplicate to estimate LoB. U stands for undetermined and marks samples with no detectable RNA above LoB. Two-sided paired T tests were performed on n = 4 independent extractions for each spike-in condition. Associated statistics are summarized in Supplementary Data . Source data are provided as a Source data file.

Journal: Nature Communications

Article Title: Standardized preservation, extraction and quantification techniques for detection of fecal SARS-CoV-2 RNA

doi: 10.1038/s41467-021-25576-6

Figure Lengend Snippet: Stool samples collected from omnivorous donors and processed into a single standardized matrix by NIST was spiked with ATCC CoV-2 RNA or BCoV vaccine. Spiked stool was preserved in the OMNIgene-GUT Kit (OG), Zymo DNA/RNA shield buffer (ZY), and PBS (as indicated in the tab on the top). RNA was extracted from these samples by two independent users, each in duplicate, using the MagMAX Viral/Pathogen Kit (MM; green), QIAamp Viral RNA Mini Kit (QA; orange), or Zymo Quick-RNA Viral Kit (ZY; purple) as indicated on the x -axis. RNA was assayed using ddPCR. a Absolute concentration of SARS-CoV-2 RNA assayed by ddPCR targeting the N1 gene is plotted on the y -axis. NIST stool matrix was spiked with 10 3 (triangle) or 10 4 (square) copies of ATCC synthetic SARS-CoV-2 RNA. b Absolute concentration of BCoV RNA assayed by ddPCR targeting the M gene is plotted on the y -axis. NIST stool matrix was spiked with 1:10 diluted (triangle) or undiluted (square) BCoV vaccine. Control samples with no spiked in RNA (none; circle) were included in duplicate to estimate LoB. U stands for undetermined and marks samples with no detectable RNA above LoB. Two-sided paired T tests were performed on n = 4 independent extractions for each spike-in condition. Associated statistics are summarized in Supplementary Data . Source data are provided as a Source data file.

Article Snippet: NIST stool matrix was spiked with 10 3 (triangle) or 10 4 (square) copies of ATCC synthetic SARS-CoV-2 RNA. b Absolute concentration of BCoV RNA assayed by ddPCR targeting the M gene is plotted on the y -axis.

Techniques: Concentration Assay, Control